A broad host range reporter plasmid for the analysis of divergent promoter regions

Jiwaji, M. and Matcher, G.F. and Dorrington, R.A. (2008) A broad host range reporter plasmid for the analysis of divergent promoter regions. South African Journal of Science, 104 (July/August). pp. 305-307. ISSN 0038-2353

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Abstract

Although many vectors exist for Escherichia coli and closely related species, there are few broad host range vectors that can be conjugated into a large variety of Gram-negative bacteria. We have constructed a broad host range vector, pMJ445, that facilitates the analysis of divergent promoters in Gram-negative bacteria. The vector was validated using two intergenic regions derived from gene clusters involved in hydantoin hydrolysis, from the environmental isolates Pseudomonas putida and Agrobacterium tumefaciens. The DNA sequences analysed were capable of activating expression of the reporter enzymes, β-glucuronidase and β-galactosidase, present on pMJ445, indicating the presence of divergent promoters in the sequences selected. In addition, we demonstrated that pMJ445 can be applied to gene regulation studies.

Item Type:Article
Uncontrolled Keywords:Escherichia coli; Plasmid DNA; Gene vector; Gram-negative bacteria
Subjects:Y Unknown > Subjects to be assigned
Divisions:Faculty > Faculty of Science > Biochemistry, Microbiology & Biotechnology
ID Code:1237
Deposited By: Ms Vivien Botha
Deposited On:04 Nov 2008
Last Modified:06 Jan 2012 16:20
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