Recent African derivation of Chrysomya putoria from C. chloropyga and mitochondrial DNA paraphyly of cytochrome oxidase subunit one in blowflies of forensic importance

Wells, J.D. and Lunt, N. and Villet, M.H. (2004) Recent African derivation of Chrysomya putoria from C. chloropyga and mitochondrial DNA paraphyly of cytochrome oxidase subunit one in blowflies of forensic importance. Medical and Veterinary Entomology, 18 (4). pp. 445-448. ISSN 0269-283X

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Official URL: http://dx.doi.org/10.1111/j.0269-283X.2004.00531.x

Abstract

Chrysomya chloropyga (Wiedemann) and C. putoria (Wiedemann) (Diptera: Calliphoridae) are closely related Afrotropical blowflies that breed in carrion and latrines, reaching high density in association with humans and spreading to other continents. In some cases of human death, Chyrsomya specimens provide forensic clues. Because the immature stages of such flies are often difficult to identify taxonomically, it is useful to develop DNA-based tests for specimen identification. Therefore we attempted to distinguish between C. chloropyga and C. putoria using mitochondrial DNA (mtDNA) sequence data from a 593-bp region of the gene for cytochrome oxidase subunit one (COI). Twelve specimens from each species yielded a total of five haplotypes, none being unique to C. putoria. Therefore it was not possible to distinguish between the two species using this locus. Maximum parsimony analysis indicated paraphyletic C. chloropyga mtDNA with C. putoria nested therein. Based on these and previously published data, we infer that C. putoria diverged very recently from C. chloropyga.

Item Type:Article
Additional Information:The definitive version is available at www.blackwell-synergy.com
Uncontrolled Keywords:blowflies; Calliphoridae; Chrysomya; Cytochrome oxidase; cytochrome c oxidase; Chrysomya chloropyga; Chrysomya putoria; Putoria; Diptera; evolution; forensic entomology; Mitochondrial DNA; molecular systematics; paraphyletic species; reciprocal mitochondrial DNA monophyly; entomology; animal; article; fly; forensic medicine; genetics; haplotype; molecular evolution; molecular genetics; nucleotide sequence; phylogeny; electron transport Complex IV; forensic Medicine; Haplotypes; molecular Sequence Data; Protein Subunits; South Africa; U.S.A.; GENBANK, AY139691(submitted), AY139692(submitted), AY139693(submitted), AY139694(submitted), AY139695(submitted); Electron Transport Complex IV, EC 1.9.3.1
Subjects:Y Unknown > Subjects to be assigned
Divisions:Faculty > Faculty of Science > Zoology & Entomology
ID Code:1278
Deposited By:INVALID USER
Deposited On:01 Feb 2009
Last Modified:06 Jan 2012 16:20
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