Stronger induction of callose deposition in barley by Russian wheat aphid than bird cherry-oat aphid is not associated with differences in callose synthase or ≤-1,3-glucanase expression.

Saheed, S. A. and Cierlik, I. and Larsson, K.A.E. and Delp, G. and Bradley, G. and Jonsson, L.M.V. and Botha, C.E.J. (2009) Stronger induction of callose deposition in barley by Russian wheat aphid than bird cherry-oat aphid is not associated with differences in callose synthase or ≤-1,3-glucanase expression. Physiologia Plantarum, 135 (2). pp. 150-161. ISSN 0031-9317

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Official URL: http://dx.doi.org/10.1111/j.1399-3054.2008.01180.x

Abstract

The effects of infestation by the bird cherry-oat aphid (BCA), (Rhopalosiphum padi L) and the Russian wheat aphid (RWA) (Diuraphis noxia Mordvilko) on callose deposition and gene expression related to callose accumulation were investigated in barley (Hordeum vulgare L. cv. Clipper). The BCA, which gives no visible symptoms, induced very limited callose deposition, even after 14 days of infestation. In contrast, RWA, which causes chlorosis, white and yellow streaking and leaf rolling, induced callose accumulation already after 24h in longitudinal leaf veins. The deposition was pronounced after 72 h, progressing during 7 and 14 days of infestation. In RWA-infested source leaves, callose was also induced in longitudinal veins basipetal to the aphid-infested tissue, whereas in sink leaves, more callose deposition was found above the feeding sites. Nine putative callose synthase genes were identified in a data base search, of which eight were expressed in the leaves, but with similar level of expression in control and aphid-infested tissue. Four out of 12 examined β-1,3-glucanases were expressed in the leaves, and three of them were up-regulated in aphid-infested tissue. They were all more strongly induced by RWA than BCA. The results suggest that callose accumulation may be partly responsible for the symptoms resulting from RWA feeding and that a callose-inducing signal may be transported in the phloem. Furthermore it is concluded that the absence of callose deposition in BCA-infested leaves is not due to a stronger induction of callose-degrading β-1,3-glucanases in this tissue, as compared to RWA-infested leaves.

Item Type:Article
Additional Information:The definitive version is available at www.blackwell-synergy.com
Uncontrolled Keywords:Russian wheat aphid, RWA, Diuraphis noxia Mordvilko, Bird cherry-oat aphid, BCA, Rhopalosiphum padi L., barley, Hordeum vulgare L. var Clipper, callose
Subjects:Y Unknown > Subjects to be assigned
Divisions:Faculty > Faculty of Science > Botany
ID Code:1298
Deposited By: Prof Ted/CEJ Botha
Deposited On:26 Feb 2009
Last Modified:01 Aug 2012 10:39
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