Suitability of total coliform beta-D-galactosidase activity and CFU counts in monitoring faecal contamination of environmental water samples

Wutor, V. C. and Togo, C.A. and Pletschke, B.I. (2009) Suitability of total coliform beta-D-galactosidase activity and CFU counts in monitoring faecal contamination of environmental water samples. Water SA, 35 (1). pp. 85-88. ISSN 0378-4738

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Abstract

Total coliforms are a group of bacteria found in high numbers in mammalian intestines; hence their presence in water indicates the possible contamination with faecal material. Total and faecal coliform counts were monitored over a period of 18 months using mFC, m-Endo and CM1046 media together with enzymatic assays on 215 environmental water samples obtained from the Eastern Cape Province of South Africa. A positive correlation, with an R2 value of 0.9393 was observed between faecal and total coliform colony units employing mFc and m-Endo media, and 0.8818 using CM1046 media. Also, a positive correlation was observed between Escherichia coli colony-forming units and β-D-galactosidase (B-GAL) activity (R2=0.8542). Overall, this study indicated that faecal contamination of environmental water samples could be monitored by measuring total coliform β-galactosidase activity and total coliform colony-forming units.

Item Type:Article
Uncontrolled Keywords:colony-forming units (CFUs); coliforms; E. coli; beta-D-galactosidase (B-GAL); beta-D-glucuronidase (GUD)
Subjects:Y Unknown > Subjects to be assigned
Divisions:Faculty > Faculty of Science > Biochemistry, Microbiology & Biotechnology
ID Code:1369
Deposited By: Mrs Eileen Shepherd
Deposited On:15 May 2009
Last Modified:01 Aug 2012 13:22
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