Not all J domains are created equal: implications for the specificity of Hsp40-Hsp70 interactions.

Hennessey, F. and Nicoll, W.S. and Zimmerman, R. and Cheetham, M.E. and Blatch, G.L. (2005) Not all J domains are created equal: implications for the specificity of Hsp40-Hsp70 interactions. Protein Science, 14 (7). pp. 1697-1709. ISSN 0961-8368

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Official URL: http://dx.doi.org/10.1110/ps.051406805

Abstract

Heat shock protein 40s (Hsp40s) and heat shock protein 70s (Hsp70s) form chaperone partnerships that are key components of cellular chaperone networks involved in facilitating the correct folding of a broad range of client proteins. While the Hsp40 family of proteins is highly diverse with multiple forms occurring in any particular cell or compartment, all its members are characterized by a J domain that directs their interaction with a partner Hsp70. Specific Hsp40-Hsp70 chaperone partnerships have been identified that are dedicated to the correct folding of distinct subsets of client proteins. The elucidation of the mechanism by which these specific Hsp40-Hsp70 partnerships are formed will greatly enhance our understanding of the way in which chaperone pathways are integrated into finely regulated protein folding networks. From in silico analyses, domain swapping and rational protein engineering experiments, evidence has accumulated that indicates that J domains contain key specificity determinants. This review will critically discuss the current understanding of the structural features of J domains that determine the specificity of interaction between Hsp40 proteins and their partner Hsp70s. We also propose a model in which the J domain is able to integrate specificity and chaperone activity.

Item Type:Article
Additional Information:Review article
Uncontrolled Keywords:J domain; DnaJ; Hsp70; specificity determinants J domain; DnaJ; Hsp70; specificity determinants DnaJ; Hsp70; J domain; Specificity determinants; protein domain; protein protein interaction; HSP40 Heat-Shock Proteins; Substrate Specificity
Subjects:Y Unknown > Subjects to be assigned
Divisions:Faculty > Faculty of Science > Biochemistry, Microbiology & Biotechnology
ID Code:1397
Deposited By: Mrs Eileen Shepherd
Deposited On:10 Jun 2009
Last Modified:06 Jan 2012 16:20
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