Heterologous expression of plasmodial proteins for structural studies and functional annotation.

Birkholtz, L-M. and Blatch, G.L. and Coetzer, T.L. and Hoppe, H.C. and Human, E. and Morris, E.J. and Nqcete, Z. and Oldfield, L. and Roth, R. and Shonhai, A. and Louw, A.I. (2008) Heterologous expression of plasmodial proteins for structural studies and functional annotation. Malaria Journal, 7 (Article number 197). ISSN 1475-2875

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Official URL: http://dx.doi.org/10.1186/1475-2875-7-197

Abstract

Malaria remains the world's most devastating tropical infectious disease with as many as 40% of the world population living in risk areas. The widespread resistance of Plasmodium parasites to the cost-effective chloroquine and antifolates has forced the introduction of more costly drug combinations, such as Coartem. In the absence of a vaccine in the foreseeable future, one strategy to address the growing malaria problem is to identify and characterize new and durable antimalarial drug targets, the majority of which are parasite proteins. Biochemical and structure-activity analysis of these proteins is ultimately essential in the characterization of such targets but requires large amounts of functional protein. Even though heterologous protein production has now become a relatively routine endeavour for most proteins of diverse origins, the functional expression of soluble plasmodial proteins is highly problematic and slows the progress of antimalarial drug target discovery. Here the status quo of heterologous production of plasmodial proteins is presented, constraints are highlighted and alternative strategies and hosts for functional expression and annotation of plasmodial proteins are reviewed.

Item Type:Article
Additional Information:Review article
Uncontrolled Keywords:plasmodium protein; protozoal protein; recombinant protein; heterologous expression; protein expression; protein function; protein structure; gene expression; Gene Expression; Protozoan Proteins; Recombinant Proteins
Subjects:Y Unknown > Subjects to be assigned
Divisions:Faculty > Faculty of Science > Biochemistry, Microbiology & Biotechnology
ID Code:1408
Deposited By: Mrs Eileen Shepherd
Deposited On:30 Jun 2009
Last Modified:06 Jan 2012 16:20
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