The cellulolytic and hemi-cellulolytic system of Bacillus licheniformis SVD1 and the evidence for production of a large multi-enzyme complex

Van Dyk, J.S. and Sakka, M. and Sakka, K. and Pletschke, B.I. (2009) The cellulolytic and hemi-cellulolytic system of Bacillus licheniformis SVD1 and the evidence for production of a large multi-enzyme complex. Enzyme and Microbial Technology, 45 (5). pp. 372-378. ISSN 0141-0229

[img]
Preview
Text
Pletschke_cellulolytic.pdf

606Kb

Official URL: http://dx.doi.org/10.1016/j.enzmictec.2009.06.016

Abstract

The cellulolytic and hemi-cellulolytic system of Bacillus licheniformis SVD1 was isolated and characterised in birchwood xylan cultures. The predominant activity in the crude culture was xylanase activity, but the crude culture also displayed Avicelase, carboxymethylcellulase (CMCase), mannanase, and pectinase activity. Most of the xylanase activity was found in the culture supernatant, but some activity was cell-associated. Using Sepharose 4B size exclusion chromatography, a 2000 kDa multi-enzyme complex (MEC) was purified. The MEC contained predominantly xylanase activity, as well as significant levels of mannanase and CMCase activity, but no Avicelase activity. SDS-PAGE revealed up to eight visible bands in the MEC while zymograms of the MEC displayed two xylanase active bands at 21 kDa and 45 kDa, and two CMCase active bands at 25 kDa and 30 kDa. More active bands were visible in the crude supernatant with an additional xylanase active band at 40 kDa and an additional CMCase active band at 55 kDa. Using thin layer chromatography (TLC), it was established that the crude fraction could release xylose from insoluble birchwood xylan, while the MEC was only able to produce xylobiose from this substrate. The MEC was further able to bind to insoluble xylan, but was unable to bind to crystalline cellulose. This MEC lacks many of the characteristic features of a cellulosome and is most likely a different type of complex. The presence of both high xylanase and mannanase activity makes this MEC unusual.

Item Type:Article
Uncontrolled Keywords:Cellulosome; Mannanase; Multi-enzyme complex; Xylanase; Zymogram
Subjects:Y Unknown > Subjects to be assigned
Divisions:Faculty > Faculty of Science > Biochemistry, Microbiology & Biotechnology
ID Code:1504
Deposited By: Mrs Eileen Shepherd
Deposited On:30 Sep 2009
Last Modified:06 Jan 2012 16:20
914 full-text download(s) since 30 Sep 2009
382 full-text download(s) in the past 12 months
More statistics...

Repository Staff Only: item control page