Simultaneous determination of irinotecan hydrochloride and its related compounds by high performance liquid chromatography using ultraviolet detection

Mohammadi, A. and Esmaeili, F and Dinarvand, R. and Atyabi, F and Walker, R.B. (2010) Simultaneous determination of irinotecan hydrochloride and its related compounds by high performance liquid chromatography using ultraviolet detection. Asian Journal of Chemistry, 22 (5). pp. 3966-3972.

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Abstract

A new simple, precise and accurate high performance liquid chromatography (HPLC) method was developed and validated for the simultaneous determination of irinotecan (CPT-11) and two related compounds viz., 7-ethyl-10 hydroxycamptothecin (SN-38) and camptothecin (CPT) in pharmaceutical dosage forms. Chromatography was accomplished using a reversed-phase C18 column and ultraviolet (UV)detection and an isocratic mobile phase consisting of 3 % v/v triethylammonium acetate buffer (pH 3) and acetonitrile (70:30 v/v). The linear range of quantitation for all the compounds was 0.1-10 μg/mL. The limit of quantitation for all the compounds ranged between 0.01-0.05 μg/mL. The method has the requisite accuracy, selectivity, sensitivity and precision to assay of CPT-11 and related compounds in pharmaceutical dosage forms and bulk API.

Item Type:Article
Uncontrolled Keywords:Irinotecan, camptothecin, high performance liquid chromatography-ultra violet, 7-Ethyl-10-hydroxycamptothecin
Subjects:R Medicine > RS Pharmacy and materia medica
Divisions:Faculty > Faculty of Pharmacy
ID Code:1728
Deposited By: Ms Thandiwe Menze
Deposited On:10 Jun 2010 09:57
Last Modified:06 Jan 2012 16:21
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