The structure and function of the gametes of the striped field mouse (Rhabdomys pumilio)

Tinney, Gregory Michael (2000) The structure and function of the gametes of the striped field mouse (Rhabdomys pumilio). Masters thesis, Rhodes University.

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Abstract

This studywas initiated inan attempt toadd the four-striped field mouse (Rhabdomys pumilio) to the small group of mammals that are used to studyand develop assisted reproductive technologies. To accomplish this, a complete knowledge of both the male and female gametes was necessary, as well as a repeatable and successful IVF protocol. The gametes of the field mouse, both the falciformspermand the mature oocytes, were shown to be similar in several respects to those of many other rodent species. The inductionof both the capacitated state and the acrosome reaction in the sperm were readily achieved. The success of retrieving mature oocytes was very low, with the rare occurrence of polar bodies within ovulated oocytes. The difficulties with oocytes was either related to the lack of a regular oestrous cycle, or to the hormonal regime used to induce superovulation. Although severalprotocols were used to accomplish superovulation in this species, it was seldom achieved. In in vitro fertilization trials, sperm were found to attach to both the cumulus oophorus and the zona pellucida of the oocytes. However, fertilization was never accomplished. This failure to achieve fertilization was probably related to the immaturity of the oocytes. Without further studies on Rhabdomys pumilio and further attempts at accomplishing in vitro fertilization, it would seemthat this specieswould not easily be introduced as an animalmodelfor assisted reproduction.

Item Type:Thesis (Masters)
Uncontrolled Keywords:Gametes, Mice, Microtus
Subjects:Q Science > QL Zoology > Chordates. Vertebrates > Mammals
Divisions:Faculty > Faculty of Science > Zoology & Entomology
ID Code:2346
Deposited By: Mrs Carol Perold
Deposited On:12 Jan 2012 06:48
Last Modified:12 Jan 2012 06:48
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