The phloem unloading and sucrose-sequestration pathway in the internodal stem tissue of the Saccharum hybrid var. NCo376

Gerber, Jacqués. (2001) The phloem unloading and sucrose-sequestration pathway in the internodal stem tissue of the Saccharum hybrid var. NCo376. Masters thesis, Rhodes University.




Internodes 5-8, 10, 13 and 15 of Saccharum sp. var. NCo376 were examined for evidence of symplasmic phloem unloading of sucrose from the phloem, via the bundle sheath to the storage parenchyma. The vascular bundle possesses wellisolated phloem comprised of large diameter sieve elements and small diameter companion cells. A layer of phloem parenchyma surrounds the phloem, except where the phloem abuts the crushed protophloem. Outside this is a sclerenchymatous sheath, directly endarch to a parenchymatous bundle sheath, which is surrounded by storage parenchyma. The bundle sheath is interrupted at the centrifugal pole of the vascular bundle by a phloem fibre cap. Scanning Electron Microscopy revealed plasmodesmal fields throughout the bundle sheath and pith tissue. Transmission Electron Microscopy studies provided evidence of plasmodesmal occlusion, but not in all tissues. Aniline blue reactions under UV light indicate the presence of occluded plasmodesmal fields at the phloem parenchyma / sclerenchymatous sheath interface, and in localised regions of cells which are smaller than the surrounding storage parenchyma cells. This suggests a symplasmic transport pathway at these locations, and, based on these positive aniline blue reactions, with regulation via callose-mediated transplasmodesmal transport. Osmotic stress experiments, which included the addition of Ca2+, did not reveal further callose occlusion in the parenchyma, suggesting that the plasmodesmata in these regions may be closed via a noncallosic mechanism. Dye-coupling studies, using Lucifer Yellow (LYCH), which was iontophoretically injected following turgor-pressure equalisation, showed only rare, limited symplastic transport, usually only between the injected cell and one adjacent cell. Most injections did not result in transport of LYCH, suggesting either a lack of plasmodesmal connectivity, occlusion, or gating of any plasmodesmata present. This limited symplasmic transport, combined with the presence of occluded plasmodesmata at the phloem parenchyma / sclerenchymatous sheath interface suggests the presence of a two-domain phloem-unloading pathway. While symplastic transport may occur from the phloem to the sclerenchymatous sheath, further sucrose transport to the storage parenchyma appears to proceed apoplasmically from the sclerenchymatous sheath / bundle sheath interface, and into storage parenchyma cells across the cell wall and cell membrane via specialised sucrose transporters

Item Type:Thesis (Masters)
Uncontrolled Keywords:Saccharum, Sugar cane, Sucrose
Subjects:Q Science > QK Botany
Divisions:Faculty > Faculty of Science > Botany
Supervisors:Botha, C.E.J.
ID Code:2945
Deposited By: Mrs Carol Perold
Deposited On:05 Jun 2012 09:05
Last Modified:05 Jun 2012 09:05
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