Novel amperometric glucose biosensor based on an ether-linked cobalt(II) phthalocyanine–cobalt(II) tetraphenylporphyrin pentamer as a redox mediator

Ozoemena, K.I. and Nyokong, T. (2006) Novel amperometric glucose biosensor based on an ether-linked cobalt(II) phthalocyanine–cobalt(II) tetraphenylporphyrin pentamer as a redox mediator. Electrochimica Acta, 51 (24). pp. 5131-5136. ISSN 0013-4686

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Official URL: http://dx.doi.org/10.1016/j.electacta.2006.03.055

Abstract

The development of cobalt(II) phthalocyanine–cobalt(II) tetra(5-phenoxy-10,15,20-triphenylporphyrin), (CoPc–(CoTPP)[subscript 4]) pentamer as a novel redox mediator for amperometric enzyme electrode sensitive to glucose is described. A glassy carbon electrode (GCE) was first modified with the pentamer, then followed by the immobilization onto the GCE–CoPc–(CoTPP)[subscript 4] with glucose oxidase (GOx) through cross-linking with glutaraldehyde in the presence of bovine serum albumin (BSA) and Nafion® cation-exchange polymer. The proposed biosensor displayed good amperometric respose charateristics to glucose in pH 7.0 PBS solution; such as low overpotentials (+400 mV versus Ag|AgCl), very fast amperometric response time (~5 s), linear concentration range extended up to 11 mM, with 10 μM detection limit. The biosensor exhibited electrochemical Michaelis–Menten kinetics and showed an average apparent Michaelis–Menten constant (K′M) of 14.91 ± 0.46 mM over a storage period of 2 weeks.

Item Type:Article
Uncontrolled Keywords:Cobalt phthalocyanine–cobalt porphyrin pentamer; Glucose; Glucose oxidase; Amperometric enzyme electrode; Mediator; Nafion®
Subjects:Y Unknown > Subjects to be assigned
Divisions:Faculty > Faculty of Science > Chemistry
ID Code:437
Deposited By:INVALID USER
Deposited On:31 Oct 2006
Last Modified:06 Jan 2012 16:18
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