Cellulases (CMCases) and polyphenol oxidases from thermophilic Bacillus spp. isolated from compost

Mayende, L. and Wilhelmi, Brendan Shane and Pletschke, B.I. (2006) Cellulases (CMCases) and polyphenol oxidases from thermophilic Bacillus spp. isolated from compost. Soil Biology and Biochemistry, 38 (9). pp. 2963-2966. ISSN 0038-0717

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Official URL: http://dx.doi.org/10.1016/j.soilbio.2006.03.019

Abstract

In composting, organic matter is degraded by cellulases and ligninolytic enzymes at temperatures typically above 50 °C. This study isolated thermophilic microorganisms from a compost system that were then screened for cellulase and polyphenol oxidase activity. Temperature optima for the cellulases and polyphenol oxidases were determined as 70 and 40 °C, respectively. Maximal cellulase activity was determined as 1.333 mg glucose released ml[superscript −1] min[superscript −1]. Maximal polyphenol oxidase activity attained was 5.111 nmol phenol ml[superscript −1] min[superscript −1]. Cellulases were found to be stable over a period of 1 h. The isolated compost microorganisms were identified as strains of Bacillus using 16S ribosomal DNA sequence analysis.

Item Type:Article
Uncontrolled Keywords:Cellulases; Compost; Polyphenol oxidases; Thermophiles
Subjects:Y Unknown > Subjects to be assigned
Divisions:Faculty > Faculty of Science > Biochemistry, Microbiology & Biotechnology
ID Code:439
Deposited By: Mrs Eileen Shepherd
Deposited On:31 Oct 2006
Last Modified:01 Aug 2012 13:17
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