The effect of sulfide on α-glucosidases: Implications for starch degradation in anaerobic bioreactors

Watson, S.D. and Pletschke, B.I. (2006) The effect of sulfide on α-glucosidases: Implications for starch degradation in anaerobic bioreactors. Chemosphere, 65 (1). pp. 159-164. ISSN 0045-6535

[img]
Preview
Text
Pletschke_The_effect_of_sulf.pdf

268Kb

Official URL: http://dx.doi.org/10.1016/j.chemosphere.2006.03.01...

Abstract

Membrane associated α-glucosidase activity was investigated in a methanogenic bioreactor (MR) and a biosulfidogenic bioreactor (SR). Temperature and pH optima studies showed temperature optima of 50 °C and pH optima of 8.0 for the α-glucosidases from both the MR and SR. Sulfide (at a concentration of 150 mg l[superscript (−1)]) resulted in the complete loss of all α-glucosidase activity in both the MR and SR. β-Glucosidase activities in our bioreactors were previously shown to be stimulated in the presence of sulfide. α-Glucosidases, in contrast, are inhibited by sulfide. This differential effect of sulfide on α-glucosidase and β-glucosidase activities is highlighted and is of crucial consequence to the respective degradation and utilization of starch and cellulose substrates in natural anaerobic environments and anaerobic bioreactors specifically designed for the accelerated digestion of wastewater sludge under biosulfidogenic conditions.

Item Type:Article
Uncontrolled Keywords:Hydrolysis; SRB; Sulfate reducers; Sulfate reduction; Sulfide
Subjects:Y Unknown > Subjects to be assigned
Divisions:Faculty > Faculty of Science > Biochemistry, Microbiology & Biotechnology
ID Code:473
Deposited By: Mrs Eileen Shepherd
Deposited On:22 Nov 2006
Last Modified:06 Jan 2012 16:18
380 full-text download(s) since 22 Nov 2006
58 full-text download(s) in the past 12 months
More statistics...

Repository Staff Only: item control page