Watson, S.D. and Pletschke, B.I. (2006) The effect of sulfide on α-glucosidases: Implications for starch degradation in anaerobic bioreactors. Chemosphere, 65 (1). pp. 159-164. ISSN 0045-6535
Official URL: http://dx.doi.org/10.1016/j.chemosphere.2006.03.01...
Membrane associated α-glucosidase activity was investigated in a methanogenic bioreactor (MR) and a biosulfidogenic bioreactor (SR). Temperature and pH optima studies showed temperature optima of 50 °C and pH optima of 8.0 for the α-glucosidases from both the MR and SR. Sulfide (at a concentration of 150 mg l[superscript (−1)]) resulted in the complete loss of all α-glucosidase activity in both the MR and SR. β-Glucosidase activities in our bioreactors were previously shown to be stimulated in the presence of sulfide. α-Glucosidases, in contrast, are inhibited by sulfide. This differential effect of sulfide on α-glucosidase and β-glucosidase activities is highlighted and is of crucial consequence to the respective degradation and utilization of starch and cellulose substrates in natural anaerobic environments and anaerobic bioreactors specifically designed for the accelerated digestion of wastewater sludge under biosulfidogenic conditions.
|Uncontrolled Keywords:||Hydrolysis; SRB; Sulfate reducers; Sulfate reduction; Sulfide|
|Subjects:||Y Unknown > Subjects to be assigned|
|Divisions:||Faculty > Faculty of Science > Biochemistry, Microbiology & Biotechnology|
|Deposited By:||Mrs Eileen Shepherd|
|Deposited On:||22 Nov 2006|
|Last Modified:||06 Jan 2012 16:18|
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