The ultrastructure and computer-enhanced digital image analysis of plasmodesmata at the Kranz mesophyll-bundle sheath interface of Themeda triandra var. imberbis (Retz) A. Camus in conventionally-fixed leaf blades.

Botha, C.E.J. and Hartley, B.J. and Cross, R.H.M. (1993) The ultrastructure and computer-enhanced digital image analysis of plasmodesmata at the Kranz mesophyll-bundle sheath interface of Themeda triandra var. imberbis (Retz) A. Camus in conventionally-fixed leaf blades. Annals of Botany, 72 . pp. 255-261. ISSN 0305-7364

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Official URL: http://aob.oxfordjournals.org/cgi/reprint/72/3/255

Abstract

The ultrastructure of the plasmodesmata at the Kranz mesophyll-bundle sheath (KMS-BS) interface in Themeda triandra, and the substructures within the plasmodesmata were investigated, using conventionally fixed leaf-blade material, enhanced by the addition of 0.1% tannic acid to the primary fixative. Examination of high-resolution electronmicrographs, and computer-enhanced digital images suggests that these plasmodesmata are complex structures, comprised of helically-arranged particulate material. The electron-dense particles are between 2.5 and 3.0nm in diameter. These particles are specifically associated with the inner face of the inner plasmalemma membrane leaflet, and the outer region of the desmotubule wall. The electron-dense particles are presumably proteinaceous and embedded in a lipid matrix. In the constricted median portion of the KMS-BS plasmodesmata, the space between the desmotubule and the inner plasmalemma membrane leaflet and areas surrounding the proteinaceous particles thereof (the cytoplasmic sleeve) is about 3nm in cross-section, and constitutes what we believe to be the space through which intercellular transport takes place.

Item Type:Article
Uncontrolled Keywords:Themeda triandra, Poaceae, cytoplasmic sleeve, image analysis, plasmodesmatal structure, desmotubule, Kranz mesophyll.
Subjects:Y Unknown > Subjects to be assigned
Divisions:Support Units > Electron Microscope Unit
Faculty > Faculty of Science > Botany
ID Code:479
Deposited By: Ms Vivien Botha
Deposited On:20 Nov 2006
Last Modified:06 Jan 2012 16:18
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