The simultaneous determination of trimethoprim, sulphamethoxazole and N4-acetylsulphamethoxazole in biological fluids by high pressure liquid chromatography

Gochin, R. and Kanfer, I. and Haigh, J.M. (1981) The simultaneous determination of trimethoprim, sulphamethoxazole and N4-acetylsulphamethoxazole in biological fluids by high pressure liquid chromatography. Journal of Chromatography B: Biomedical Sciences and Applications, 223 (1). pp. 139-145. ISSN 1570-0232

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Official URL: http://dx.doi.org/10.1016/S0378-4347(00)80076-6

Abstract

The simultaneous determination of trimethoprim, sulphamethoxazole and N4-acetylsulphamethoxazole in serum and urine by high-performance liquid chromatography using sulphafurazole as internal standard is described. The separation was achieved on a reversed-phase column employing acetic acid-methanol as the mobile phase with spectrophotometric detection at 230 nm. Precise simultaneous quantitative analysis of the relative components has been achieved at levels of 0.1 μg/ml for both sulphamethoxazole and its N4-acetyl metabolite using 1 ml of serum or urine.

Item Type:Article
Uncontrolled Keywords:n4 acetylsulfamethoxazole; sulfamethoxazole; trimethoprim; acetylsulfamethoxazole; drug determination; drug mixture; high performance liquid chromatography; human; serum; urine
Subjects:Y Unknown > Subjects to be assigned
Divisions:Faculty > Faculty of Pharmacy
ID Code:596
Deposited By: Prof John Haigh
Deposited On:31 May 2007
Last Modified:06 Jan 2012 16:18
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