Determination of phenylpropanolamine in serum and urine by high performance liquid chromatography

Dowse, R. and Haigh, J.M. and Kanfer, I. (1983) Determination of phenylpropanolamine in serum and urine by high performance liquid chromatography. Journal of Pharmaceutical Sciences, 72 (9). pp. 1018-1020. ISSN 0022-3549

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Official URL: http://dx.doi.org/10.1002/jps.2600720912

Abstract

A high-performance liquid chromatographic analysis of phenylpropanolamine in human serum and urine without prior derivatization is presented. Using direct UV detection the method is sufficiently sensitive to detect 25 ng of drug/ml of serum or urine; the coefficients of variation at 25 ng/ml and 500 ng/ml were 5.16 and 2.12, respectively, in serum. The method involves serum and urine extraction at a basic pH with chloroform, a single back-extraction, and chromatography on a reverse-phase column. Serum and urine data following administration of a single 150-mg sustained-release tablet of phenylpropanolamine hydrochloride in 6 healthy volunteers demonstrates the suitability of the analytical method.

Item Type:Article
Uncontrolled Keywords:Phenylpropanolamine - determination by high-performance liquid chromatography without prior derivatization; human serum and urine • High-performance liquid chromatography - phenylpropanolamine; determination in human serum and urine without prior derivatization
Subjects:Y Unknown > Subjects to be assigned
Divisions:Faculty > Faculty of Pharmacy
ID Code:602
Deposited By: Prof John Haigh
Deposited On:31 May 2007
Last Modified:06 Jan 2012 16:18
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