High performance liquid chromatographic analysis of oleandomycin in serum and urine

Stubbs, C. and Haigh, J.M. and Kanfer, I. (1986) High performance liquid chromatographic analysis of oleandomycin in serum and urine. Journal of Chromatography, 353 . pp. 33-38. ISSN 0021-9673



Official URL: http://dx.doi.org/doi:10.1016/S0021-9673(01)87074-...


The determination of oleandomycin in serum and urine by high-performance liquid chromatography using erythromycin as internal standard is described. The separation was achieved on a reversed-phase C 1 s column employing acetonitrile-0.05 A4 phosphate buffer (30:70), adjusted to pH 7.0, as the mobile phase with UV detection at 200 nm. A solid-phase extraction procedure, combined with a simple phaseseparation step was used prior to chromatographic analysis. Linear calibration curves were obtained in the concentration ranges 0.25-5.0 pg/ml (serum) and 1 .O-25.0 pg/ml (urine). Precise quantitative analysis has been achieved at these levels with relative standard deviations of < 5%.

Item Type:Article
Uncontrolled Keywords:Oleandomycin/*analysis Chromatography, High Pressure Liquid; Erythromycin/analysis; Humans; Indicators and Reagents; Oleandomycin/blood; Oleandomycin/urine; Spectrophotometry, Ultraviolet
Subjects:Y Unknown > Subjects to be assigned
Divisions:Faculty > Faculty of Pharmacy
ID Code:615
Deposited By: Prof John Haigh
Deposited On:31 May 2007
Last Modified:06 Jan 2012 16:18
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