Stubbs, C. and Haigh, J.M. and Kanfer, I. (1986) High performance liquid chromatographic analysis of oleandomycin in serum and urine. Journal of Chromatography, 353 . pp. 33-38. ISSN 0021-9673
Official URL: http://dx.doi.org/doi:10.1016/S0021-9673(01)87074-...
The determination of oleandomycin in serum and urine by high-performance liquid chromatography using erythromycin as internal standard is described. The separation was achieved on a reversed-phase C 1 s column employing acetonitrile-0.05 A4 phosphate buffer (30:70), adjusted to pH 7.0, as the mobile phase with UV detection at 200 nm. A solid-phase extraction procedure, combined with a simple phaseseparation step was used prior to chromatographic analysis. Linear calibration curves were obtained in the concentration ranges 0.25-5.0 pg/ml (serum) and 1 .O-25.0 pg/ml (urine). Precise quantitative analysis has been achieved at these levels with relative standard deviations of < 5%.
|Uncontrolled Keywords:||Oleandomycin/*analysis Chromatography, High Pressure Liquid; Erythromycin/analysis; Humans; Indicators and Reagents; Oleandomycin/blood; Oleandomycin/urine; Spectrophotometry, Ultraviolet|
|Subjects:||Y Unknown > Subjects to be assigned|
|Divisions:||Faculty > Faculty of Pharmacy|
|Deposited By:||Prof John Haigh|
|Deposited On:||31 May 2007|
|Last Modified:||06 Jan 2012 16:18|
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