A novel biosensor for the detection and monitoring of β-d-galactosidase of faecal origin in water

Wutor, V. C. and Togo, C.A. and Limson, J.L. and Pletschke, B.I. (2007) A novel biosensor for the detection and monitoring of β-d-galactosidase of faecal origin in water. Enzyme and Microbial Technology, 40 (6). pp. 1512-1517. ISSN 0141-0229

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Official URL: http://dx.doi.org/10.1016/j.enzmictec.2006.10.039

Abstract

A voltammetric sensor prepared by the immobilization of metallophthalocyanine complexes onto a glassy carbon electrode has been developed for the detection of β-d-galactosidase (B-GAL) of faecal origin in water. Electrooxidation of chlorophenol red, a breakdown product of the chromogenic substrate chlorophenol red β-d-galactopyranoside, was used as a measure of β-d-galactosidase activity. At metallophthalocyanine modified electrodes, in particular copper(II) phthalocyanine, a decrease in electrode fouling was observed. The sensor was sensitive to fluctuations in pH, not significantly affected by temperature variations and could detect one colony forming unit/100 mL in 15 min. Loss of 40% sensitivity was observed over a period of 30 days. A strong correlation between sensor sensitivity and colony forming units was observed. The sensor is capable of detecting viable but nonculturable bacteria, overcoming this drawback of the use of culture media for detection of coliforms.

Item Type:Article
Uncontrolled Keywords:Chlorophenol red; Chlorophenol red β-d-galactopyranoside; Coliforms; Voltammetry; Phthalocyanine; Biosensor
Subjects:Y Unknown > Subjects to be assigned
Divisions:Faculty > Faculty of Science > Biochemistry, Microbiology & Biotechnology
ID Code:653
Deposited By: Mrs Eileen Shepherd
Deposited On:15 Jun 2007
Last Modified:01 Aug 2012 13:21
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