Sensitive high-performance liquid chromatographic determination of cyclizine and its demethylated metabolite, norcyclizine, in biological fluids using coulometric detection

Walker, R.B. and Kanfer, I. (1995) Sensitive high-performance liquid chromatographic determination of cyclizine and its demethylated metabolite, norcyclizine, in biological fluids using coulometric detection. Journal of Chromatography B: Biomedical Applications, 672 (1). pp. 172-177. ISSN 1570-0232

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Official URL: http://dx.doi.org/10.1016/0378-4347(95)00202-T

Abstract

An accurate, sensitive, selective and reproducible high-performance liquid chromatographic method with coulometric detection for the determination of cyclizine and its inactive demethylated metabolite, norcyclizine, in biological fluids has been developed. The drugs were separated using a custom packed reversed-phase C18 analytical column and phosphate buffer (0.05 M, pH 3)-acetonitrile (7:3) as mobile phase. The dual electrode coulometric detector was operated in the "oxidative-screen" mode with the upstream electrode (detector 1) set at 0.55 V and the downstream electrode (detector 2) set at 0.90 V. Serum and urine samples were prepared for analysis by solid-phase extraction, followed by a simple phase-separation step. The limit of quantitation was 1 ng/ml for both cyclizine and norcyclizine in serum and urine.

Item Type:Article
Additional Information:Short Communication
Uncontrolled Keywords:cyclizine; drug metabolite; drug blood level; drug determination; drug urine level; Chromatography, High Pressure Liquid
Subjects:Y Unknown > Subjects to be assigned
Divisions:Faculty > Faculty of Pharmacy
ID Code:768
Deposited By: Mrs Eileen Shepherd
Deposited On:03 Aug 2007
Last Modified:06 Jan 2012 16:19
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