6-Hydroxymelatonin protects against cyanide induced oxidative stress in rat brain homogenates

Maharaj, D.S. and Walker, R.B. and Glass, B.D. and Daya, S. (2003) 6-Hydroxymelatonin protects against cyanide induced oxidative stress in rat brain homogenates. Journal of Chemical Neuroanatomy, 26 (2). pp. 103-107. ISSN 0891-0618



Official URL: http://dx.doi.org/10.1016/S0891-0618(03)00034-6


Both 6-hydroxymelatonin and N-acetyl-N-formyl-5-methoxykynurenamine are photodegradants and enzymatic metabolites of melatonin and are known to retain equipotent activity against potassium cyanide-induced superoxide generation compared to melatonin. It is not clear whether one or both of these metabolites is responsible for this effect. The present study therefore investigates the possible manner in which 6-hydroxymelatonin protects against oxidative stress induced by cyanide in rat brain homogenates. We examined the ability of 6-hydroxymelatonin to scavenge KCN-induced superoxide anion generation as well as lipid peroxidation. In addition, we also examined the effect of this indole on lactate dehydrogenase activity (LDH) as well as mitochondrial electron transport using dichlorophenol–indophenol as an electron acceptor. The results of this study show that 6-hydroxymelatonin significantly reduces KCN-induced superoxide anion generation, which is accompanied by a commensurate reduction in lipid peroxidation. Partial reversal of the KCN-induced reduction in mitochondrial electron transport is accompanied by a similar reversal of mitochondrial LDH activity blunted by KCN. It can thus be proposed that 6-hydroxymelatonin is potentially neuroprotective against KCN-induced neurotoxicity.

Item Type:Article
Uncontrolled Keywords:6-Hydroxymelatonin; Electron transport chain; Free radicals; Lipid peroxidation; Mitochondria; Neurotoxicity; brain homogenate; neuroprotection; oxidative stress Superoxide anion
Subjects:Y Unknown > Subjects to be assigned
Divisions:Faculty > Faculty of Pharmacy
ID Code:785
Deposited By: Mrs Eileen Shepherd
Deposited On:21 Aug 2007
Last Modified:06 Jan 2012 16:19
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