The effect of physico-chemical parameters and chemical compounds on the activity of β-d-galactosidase (B-GAL), a marker enzyme for indicator microorganisms in water

Wutor, V. C. and Togo, C.A. and Pletschke, B.I. (2007) The effect of physico-chemical parameters and chemical compounds on the activity of β-d-galactosidase (B-GAL), a marker enzyme for indicator microorganisms in water. Chemosphere, 68 (4). pp. 622-627. ISSN 0045-6535

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Official URL: http://dx.doi.org/10.1016/j.chemosphere.2007.02.05...

Abstract

The presence of coliforms in polluted water was determined enzymatically (in situ) by directly monitoring the activity of β-d-galactosidase (B-GAL) through the hydrolysis of the yellow chromogenic subtrate, chlorophenol red β-d-galactopyranoside (CPRG), which produced a red chlorophenol red (CPR) product. The objectives of this study were to monitor the effect of compounds commonly found in the environment and used in water treatment on a B-GAL CPRG assay and to investigate the differences between the environmental B-GAL enzyme and the pure commercial enzyme. Environmental B-GAL was optimally active at pH 7.8. Two temperature optima were observed at 35 and 55 °C, respectively. B-GAL activity was strongly inhibited by silver and copper ions. While calcium and ferrous ions at lower concentrations (50-100 mg l-1) increased the enzyme activity, a reduction was observed at higher concentrations (200 mg l-1). Sodium hypochlorite, normally used in rural areas to disinfect water gradually decreased B-GAL activity at concentrations between 0 and 5600 ppm for both the commercial and environmental enzymes. B-GAL from the environment behaved differently from its commercially available counterpart.

Item Type:Article
Uncontrolled Keywords:β-d-Galactosidase; Chlorophenol red (CPR); Chlorophenol-red-β-d-galactopyranoside (CPRG); Coliforms; Faecal
Subjects:Y Unknown > Subjects to be assigned
Divisions:Faculty > Faculty of Science > Biochemistry, Microbiology & Biotechnology
ID Code:786
Deposited By: Mrs Eileen Shepherd
Deposited On:22 Aug 2007
Last Modified:01 Aug 2012 13:22
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