A capillary zone electrophoresis (CZE) method for the determination of cyclizine hydrochloride in tablets and suppositories

Mohammadi, I. and Kanfer, I. and Walker, R.B. (2004) A capillary zone electrophoresis (CZE) method for the determination of cyclizine hydrochloride in tablets and suppositories. Journal of Pharmaceutical and Biomedical Analysis, 35 (1). pp. 233-239. ISSN 0731-7085

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Official URL: http://dx.doi.org/10.1016/j.jpba.2004.01.011

Abstract

Current compendial methods of assay for the analysis of cyclizine tablets involve the use of UV spectrophotometry. Since this is a non-selective technique its application to more complex dosage forms, such as suppositories, is unlikely to be appropriate. There is therefore a need for the development of a highly specific quantitative analytical method, such as high performance liquid chromatography (HPLC) or capillary electrophoresis (CE). The latter technique was chosen in view of some specific advantages over HPLC, such as the use of relatively non-toxic aqueous buffers, as opposed to organic solvents, which obviates the use of expensive HPLC grade solvents making CE more cost effective. Cyclizine was analyzed in 50 mM phosphate buffer (pH 2.3) and run at an applied voltage 25 kV. Detection sensitivity was enhanced by using a wavelength of 200 nm and samples were loaded hydrodynamically onto an uncoated fused-silica capillary (60 cm×50 mm i.d.). Chlorcyclizine was used as the internal standard and resolution of both compounds was achieved in less than 7 min. Stress testing was undertaken in order to investigate the appearance of breakdown products. The method has the requisite accuracy, selectivity, sensitivity and precision to assay cyclizine in tablets and suppositories. Degradation products resulting from the stress studies did not interfere with the detection of cyclizine and the assay is thus stability-indicating.

Item Type:Article
Additional Information:Short communication
Uncontrolled Keywords:Chlorcyclizine; Cyclizine; CZE; Stability-indicating; Suppositories; Tablets
Subjects:Y Unknown > Subjects to be assigned
Divisions:Faculty > Faculty of Pharmacy
ID Code:789
Deposited By: Mrs Eileen Shepherd
Deposited On:23 Aug 2007
Last Modified:06 Jan 2012 16:19
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